Fluorescence detection of single nucleotide polymorphisms using a universal molecular beacon

We present a simple and novel assay—employing a universal molecular beacon (MB) in the presence of Hg(2+)—for the detection of single nucleotide polymorphisms (SNPs) based on Hg(2+)–DNA complexes inducing a conformational change in the MB. The MB (T(7)-MB) contains a 19-mer loop and a stem of a pair...

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Detalles Bibliográficos
Autores principales: Lin, Yang-Wei, Ho, Hsin-Tsung, Huang, Chih-Ching, Chang, Huan-Tsung
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2577337/
https://www.ncbi.nlm.nih.gov/pubmed/18753149
http://dx.doi.org/10.1093/nar/gkn537

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2577337/
https://www.ncbi.nlm.nih.gov/pubmed/18753149
http://dx.doi.org/10.1093/nar/gkn537

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