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Fluorescence detection of single nucleotide polymorphisms using a universal molecular beacon

We present a simple and novel assay—employing a universal molecular beacon (MB) in the presence of Hg(2+)—for the detection of single nucleotide polymorphisms (SNPs) based on Hg(2+)–DNA complexes inducing a conformational change in the MB. The MB (T(7)-MB) contains a 19-mer loop and a stem of a pair...

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Detalles Bibliográficos
Autores principales:	Lin, Yang-Wei, Ho, Hsin-Tsung, Huang, Chih-Ching, Chang, Huan-Tsung
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2008
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2577337/ https://www.ncbi.nlm.nih.gov/pubmed/18753149 http://dx.doi.org/10.1093/nar/gkn537

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