Multi-stringency wash of partially hybridized 60-mer probes reveals that the stringency along the probe decreases with distance from the microarray surface

Here, we describe a multi-parametric study of DNA hybridization to probes with 20–70% G + C content. Probes were designed towards 71 different sites/mutations in the phenylalanine hydroxylase gene. Seven probe lengths, three spacer lengths and six stringencies were systematically varied. The three s...

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Detalles Bibliográficos
Autores principales: Poulsen, Lena, Søe, Martin Jensen, Snakenborg, Detlef, Møller, Lisbeth Birk, Dufva, Martin
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2582620/
https://www.ncbi.nlm.nih.gov/pubmed/18805905
http://dx.doi.org/10.1093/nar/gkn600

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2582620/
https://www.ncbi.nlm.nih.gov/pubmed/18805905
http://dx.doi.org/10.1093/nar/gkn600

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