An Infinitely Expandable Cloning Strategy plus Repeat-Proof PCR for Working with Multiple shRNA

Vector construction with restriction enzymes (REs) typically involves the ligation of a digested donor fragment (insert) to a reciprocally digested recipient fragment (vector backbone). Creating a suitable cloning plan becomes increasingly difficult for complex strategies requiring repeated insertio...

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Detalles Bibliográficos
Autores principales: Mcintyre, Glen John, Groneman, Jennifer Lynne, Tran, Anna, Applegate, Tanya Lynn
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2584233/
https://www.ncbi.nlm.nih.gov/pubmed/19043584
http://dx.doi.org/10.1371/journal.pone.0003827

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2584233/
https://www.ncbi.nlm.nih.gov/pubmed/19043584
http://dx.doi.org/10.1371/journal.pone.0003827

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