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An Infinitely Expandable Cloning Strategy plus Repeat-Proof PCR for Working with Multiple shRNA
Vector construction with restriction enzymes (REs) typically involves the ligation of a digested donor fragment (insert) to a reciprocally digested recipient fragment (vector backbone). Creating a suitable cloning plan becomes increasingly difficult for complex strategies requiring repeated insertio...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2008
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2584233/ https://www.ncbi.nlm.nih.gov/pubmed/19043584 http://dx.doi.org/10.1371/journal.pone.0003827 |