Construction of recB-recD genetic fusion and functional analysis of RecBDC fusion enzyme in Escherichia coli

BACKGROUND: recD, located between recB and argA, encodes the smallest polypeptide (60 kDa) of the heterotrimeric enzyme RecBCD in Escherichia coli. RecD is a 5'-3' helicase and is required for the nuclease activity of RecBCD and for tight binding to dsDNA ends. Here, we have tested the hyp...

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Detalles Bibliográficos
Autores principales: Portakal, Oytun, Doğan, Pakize
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2586629/
https://www.ncbi.nlm.nih.gov/pubmed/18847457
http://dx.doi.org/10.1186/1471-2091-9-27

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2586629/
https://www.ncbi.nlm.nih.gov/pubmed/18847457
http://dx.doi.org/10.1186/1471-2091-9-27

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