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Construction of recB-recD genetic fusion and functional analysis of RecBDC fusion enzyme in Escherichia coli
BACKGROUND: recD, located between recB and argA, encodes the smallest polypeptide (60 kDa) of the heterotrimeric enzyme RecBCD in Escherichia coli. RecD is a 5'-3' helicase and is required for the nuclease activity of RecBCD and for tight binding to dsDNA ends. Here, we have tested the hyp...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
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BioMed Central
2008
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2586629/ https://www.ncbi.nlm.nih.gov/pubmed/18847457 http://dx.doi.org/10.1186/1471-2091-9-27 |