Critical evaluation of methods used to determine amplification efficiency refutes the exponential character of real-time PCR

BACKGROUND: The challenge of determining amplification efficiency has long been a predominant aspect of implementing real-time qPCR, playing a critical role in the accuracy and reliability that can be achieved. Based upon analysis of amplification profile position, standard curves are currently the...

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Detalles Bibliográficos
Autores principales: Rutledge, Robert G, Stewart, Don
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2587475/
https://www.ncbi.nlm.nih.gov/pubmed/18973660
http://dx.doi.org/10.1186/1471-2199-9-96

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2587475/
https://www.ncbi.nlm.nih.gov/pubmed/18973660
http://dx.doi.org/10.1186/1471-2199-9-96

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