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Critical evaluation of methods used to determine amplification efficiency refutes the exponential character of real-time PCR
BACKGROUND: The challenge of determining amplification efficiency has long been a predominant aspect of implementing real-time qPCR, playing a critical role in the accuracy and reliability that can be achieved. Based upon analysis of amplification profile position, standard curves are currently the...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2008
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2587475/ https://www.ncbi.nlm.nih.gov/pubmed/18973660 http://dx.doi.org/10.1186/1471-2199-9-96 |