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The antiretroviral potency of APOBEC1 deaminase from small animal species

Although the role of the APOBEC3-dependent retroelement restriction system as an intrinsic immune defense against human immunodeficiency virus type1 (HIV-1) infection is becoming clear, only the rat ortholog of mammalian APOBEC1s (A1) thus far has been shown to possess antiviral activity. Here, we c...

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Autores principales: Ikeda, Terumasa, Ohsugi, Takeo, Kimura, Tetsuya, Matsushita, Shuzo, Maeda, Yosuke, Harada, Shinji, Koito, Atsushi
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2588513/
https://www.ncbi.nlm.nih.gov/pubmed/18971252
http://dx.doi.org/10.1093/nar/gkn802
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author Ikeda, Terumasa
Ohsugi, Takeo
Kimura, Tetsuya
Matsushita, Shuzo
Maeda, Yosuke
Harada, Shinji
Koito, Atsushi
author_facet Ikeda, Terumasa
Ohsugi, Takeo
Kimura, Tetsuya
Matsushita, Shuzo
Maeda, Yosuke
Harada, Shinji
Koito, Atsushi
author_sort Ikeda, Terumasa
collection PubMed
description Although the role of the APOBEC3-dependent retroelement restriction system as an intrinsic immune defense against human immunodeficiency virus type1 (HIV-1) infection is becoming clear, only the rat ortholog of mammalian APOBEC1s (A1) thus far has been shown to possess antiviral activity. Here, we cloned A1 cDNAs from small animal species, and showed that similar to rat A1, both wild-type and Δvif HIV-1 infection was inhibited by mouse and hamster A1 (4- to 10-fold), whereas human A1 had negligible effects. Moreover, rabbit A1 significantly reduced the infectivity of both HIV-1 virions (>300-fold), as well as that of SIVmac, SIVagm, FIV and murine leukemia virus. Immunoblot analysis showed that A1s were efficiently incorporated into the HIV-1 virion, and their packaging is mediated through an interaction with the nucleocapsid Gag domain. Interestingly, there was a clear accumulation of particular C-T changes in the genomic RNAs of HIV-1 produced in their presence, with few G-A changes in the proviral DNA. Together, these data reveal that A1 may function as a defense mechanism, regulating retroelements in a wide range of mammalian species.
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spelling pubmed-25885132009-03-04 The antiretroviral potency of APOBEC1 deaminase from small animal species Ikeda, Terumasa Ohsugi, Takeo Kimura, Tetsuya Matsushita, Shuzo Maeda, Yosuke Harada, Shinji Koito, Atsushi Nucleic Acids Res Molecular Biology Although the role of the APOBEC3-dependent retroelement restriction system as an intrinsic immune defense against human immunodeficiency virus type1 (HIV-1) infection is becoming clear, only the rat ortholog of mammalian APOBEC1s (A1) thus far has been shown to possess antiviral activity. Here, we cloned A1 cDNAs from small animal species, and showed that similar to rat A1, both wild-type and Δvif HIV-1 infection was inhibited by mouse and hamster A1 (4- to 10-fold), whereas human A1 had negligible effects. Moreover, rabbit A1 significantly reduced the infectivity of both HIV-1 virions (>300-fold), as well as that of SIVmac, SIVagm, FIV and murine leukemia virus. Immunoblot analysis showed that A1s were efficiently incorporated into the HIV-1 virion, and their packaging is mediated through an interaction with the nucleocapsid Gag domain. Interestingly, there was a clear accumulation of particular C-T changes in the genomic RNAs of HIV-1 produced in their presence, with few G-A changes in the proviral DNA. Together, these data reveal that A1 may function as a defense mechanism, regulating retroelements in a wide range of mammalian species. Oxford University Press 2008-12 2008-10-29 /pmc/articles/PMC2588513/ /pubmed/18971252 http://dx.doi.org/10.1093/nar/gkn802 Text en © 2008 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Molecular Biology
Ikeda, Terumasa
Ohsugi, Takeo
Kimura, Tetsuya
Matsushita, Shuzo
Maeda, Yosuke
Harada, Shinji
Koito, Atsushi
The antiretroviral potency of APOBEC1 deaminase from small animal species
title The antiretroviral potency of APOBEC1 deaminase from small animal species
title_full The antiretroviral potency of APOBEC1 deaminase from small animal species
title_fullStr The antiretroviral potency of APOBEC1 deaminase from small animal species
title_full_unstemmed The antiretroviral potency of APOBEC1 deaminase from small animal species
title_short The antiretroviral potency of APOBEC1 deaminase from small animal species
title_sort antiretroviral potency of apobec1 deaminase from small animal species
topic Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2588513/
https://www.ncbi.nlm.nih.gov/pubmed/18971252
http://dx.doi.org/10.1093/nar/gkn802
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