Rapid and reliable universal cloning of influenza A virus genes by target-primed plasmid amplification

Reverse genetics has become pivotal in influenza virus research relying on rapid generation of tailored recombinant influenza viruses. They are rescued from transfected plasmids encoding the eight influenza virus gene segments, which have been cloned using restriction endonucleases and DNA ligation....

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Detalles Bibliográficos
Autores principales: Stech, Jürgen, Stech, Olga, Herwig, Astrid, Altmeppen, Hermann, Hundt, Jana, Gohrbandt, Sandra, Kreibich, Anne, Weber, Siegfried, Klenk, Hans-Dieter, Mettenleiter, Thomas C.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2588516/
https://www.ncbi.nlm.nih.gov/pubmed/18832366
http://dx.doi.org/10.1093/nar/gkn646

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2588516/
https://www.ncbi.nlm.nih.gov/pubmed/18832366
http://dx.doi.org/10.1093/nar/gkn646

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