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Rapid and reliable universal cloning of influenza A virus genes by target-primed plasmid amplification
Reverse genetics has become pivotal in influenza virus research relying on rapid generation of tailored recombinant influenza viruses. They are rescued from transfected plasmids encoding the eight influenza virus gene segments, which have been cloned using restriction endonucleases and DNA ligation....
Autores principales: | Stech, Jürgen, Stech, Olga, Herwig, Astrid, Altmeppen, Hermann, Hundt, Jana, Gohrbandt, Sandra, Kreibich, Anne, Weber, Siegfried, Klenk, Hans-Dieter, Mettenleiter, Thomas C. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2588516/ https://www.ncbi.nlm.nih.gov/pubmed/18832366 http://dx.doi.org/10.1093/nar/gkn646 |
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