Preferential Inhibition of Herpes-Group Viruses by Phosphonoacetic Acid: Effect on Virus DNA Synthesis and Virus-Induced DNA Polymerase Activity

In tissue culture phosphonoacetic acid (PAA) specifically inhibited DNA synthesis of human cytomegalovirus (CMV), murine CMV, simian CMV, Epstein-Barr virus, and Herpesvirus saimiri. Fifty to one hundred micrograms per milliliter PAA completely inhibited viral DNA synthesis with no significant damag...

Descripción completa

Detalles Bibliográficos
Autores principales: Huang, Eng-Shang, Huang, Chien-Hui, Huong, Shu-Mei, Selgrade, Maryjane
Formato: Texto
Lenguaje:English
Publicado: 1976
Materias:
Original Contributions
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2595326/
https://www.ncbi.nlm.nih.gov/pubmed/960726
_version_ 1782161668329963520
author Huang, Eng-Shang
Huang, Chien-Hui
Huong, Shu-Mei
Selgrade, Maryjane
author_facet Huang, Eng-Shang
Huang, Chien-Hui
Huong, Shu-Mei
Selgrade, Maryjane
author_sort Huang, Eng-Shang
collection PubMed
description In tissue culture phosphonoacetic acid (PAA) specifically inhibited DNA synthesis of human cytomegalovirus (CMV), murine CMV, simian CMV, Epstein-Barr virus, and Herpesvirus saimiri. Fifty to one hundred micrograms per milliliter PAA completely inhibited viral DNA synthesis with no significant damage to host cell DNA synthesis. In vitro DNA polymerization assays showed that 10 μg/ml of PAA specifically inhibited partially purified human CMV-induced DNA polymerase, while little inhibition of host-cell DNA polymerase activity was found. The specific inhibition of herpes-group virus DNA synthesis with little toxicity to host cells suggests that PAA has great potential as an antiherpesvirus therapeutic agent.
format Text
id pubmed-2595326
institution National Center for Biotechnology Information
language English
publishDate 1976
record_format MEDLINE/PubMed
spelling pubmed-25953262008-12-05 Preferential Inhibition of Herpes-Group Viruses by Phosphonoacetic Acid: Effect on Virus DNA Synthesis and Virus-Induced DNA Polymerase Activity Huang, Eng-Shang Huang, Chien-Hui Huong, Shu-Mei Selgrade, Maryjane Yale J Biol Med Original Contributions In tissue culture phosphonoacetic acid (PAA) specifically inhibited DNA synthesis of human cytomegalovirus (CMV), murine CMV, simian CMV, Epstein-Barr virus, and Herpesvirus saimiri. Fifty to one hundred micrograms per milliliter PAA completely inhibited viral DNA synthesis with no significant damage to host cell DNA synthesis. In vitro DNA polymerization assays showed that 10 μg/ml of PAA specifically inhibited partially purified human CMV-induced DNA polymerase, while little inhibition of host-cell DNA polymerase activity was found. The specific inhibition of herpes-group virus DNA synthesis with little toxicity to host cells suggests that PAA has great potential as an antiherpesvirus therapeutic agent. 1976-03 /pmc/articles/PMC2595326/ /pubmed/960726 Text en
spellingShingle Original Contributions
Huang, Eng-Shang
Huang, Chien-Hui
Huong, Shu-Mei
Selgrade, Maryjane
Preferential Inhibition of Herpes-Group Viruses by Phosphonoacetic Acid: Effect on Virus DNA Synthesis and Virus-Induced DNA Polymerase Activity
title Preferential Inhibition of Herpes-Group Viruses by Phosphonoacetic Acid: Effect on Virus DNA Synthesis and Virus-Induced DNA Polymerase Activity
title_full Preferential Inhibition of Herpes-Group Viruses by Phosphonoacetic Acid: Effect on Virus DNA Synthesis and Virus-Induced DNA Polymerase Activity
title_fullStr Preferential Inhibition of Herpes-Group Viruses by Phosphonoacetic Acid: Effect on Virus DNA Synthesis and Virus-Induced DNA Polymerase Activity
title_full_unstemmed Preferential Inhibition of Herpes-Group Viruses by Phosphonoacetic Acid: Effect on Virus DNA Synthesis and Virus-Induced DNA Polymerase Activity
title_short Preferential Inhibition of Herpes-Group Viruses by Phosphonoacetic Acid: Effect on Virus DNA Synthesis and Virus-Induced DNA Polymerase Activity
title_sort preferential inhibition of herpes-group viruses by phosphonoacetic acid: effect on virus dna synthesis and virus-induced dna polymerase activity
topic Original Contributions
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2595326/
https://www.ncbi.nlm.nih.gov/pubmed/960726
work_keys_str_mv AT huangengshang preferentialinhibitionofherpesgroupvirusesbyphosphonoaceticacideffectonvirusdnasynthesisandvirusinduceddnapolymeraseactivity
AT huangchienhui preferentialinhibitionofherpesgroupvirusesbyphosphonoaceticacideffectonvirusdnasynthesisandvirusinduceddnapolymeraseactivity
AT huongshumei preferentialinhibitionofherpesgroupvirusesbyphosphonoaceticacideffectonvirusdnasynthesisandvirusinduceddnapolymeraseactivity
AT selgrademaryjane preferentialinhibitionofherpesgroupvirusesbyphosphonoaceticacideffectonvirusdnasynthesisandvirusinduceddnapolymeraseactivity

Ejemplares similares