Sample prep for proteomics of breast cancer: proteomics and gene ontology reveal dramatic differences in protein solubilization preferences of radioimmunoprecipitation assay and urea lysis buffers

BACKGROUND: An important step in the proteomics of solid tumors, including breast cancer, consists of efficiently extracting most of proteins in the tumor specimen. For this purpose, Radio-Immunoprecipitation Assay (RIPA) buffer is widely employed. RIPA buffer's rapid and highly efficient cell...

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Detalles Bibliográficos
Autor principal: Ngoka, Lambert CM
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2600628/
https://www.ncbi.nlm.nih.gov/pubmed/18950484
http://dx.doi.org/10.1186/1477-5956-6-30

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2600628/
https://www.ncbi.nlm.nih.gov/pubmed/18950484
http://dx.doi.org/10.1186/1477-5956-6-30

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