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Homologous recombination-mediated cloning and manipulation of genomic DNA regions using Gateway and recombineering systems

BACKGROUND: Employing genomic DNA clones to characterise gene attributes has several advantages over the use of cDNA clones, including the presence of native transcription and translation regulatory sequences as well as a representation of the complete repertoire of potential splice variants encoded...

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Detalles Bibliográficos
Autores principales: Rozwadowski, Kevin, Yang, Wen, Kagale, Sateesh
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2601046/
https://www.ncbi.nlm.nih.gov/pubmed/19014699
http://dx.doi.org/10.1186/1472-6750-8-88