A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality

A novel method to efficiently generate unmarked in-frame gene deletions in Rhodococcus equi was developed, exploiting the cytotoxic effect of 5-fluorocytosine (5-FC) by the action of cytosine deaminase (CD) and uracil phosphoribosyltransferase (UPRT) enzymes. The opportunistic, intracellular pathoge...

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Detalles Bibliográficos
Autores principales: van der Geize, R., de Jong, W., Hessels, G. I., Grommen, A. W. F., Jacobs, A. A. C., Dijkhuizen, L.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2602762/
https://www.ncbi.nlm.nih.gov/pubmed/18984616
http://dx.doi.org/10.1093/nar/gkn811

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2602762/
https://www.ncbi.nlm.nih.gov/pubmed/18984616
http://dx.doi.org/10.1093/nar/gkn811

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