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A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality
A novel method to efficiently generate unmarked in-frame gene deletions in Rhodococcus equi was developed, exploiting the cytotoxic effect of 5-fluorocytosine (5-FC) by the action of cytosine deaminase (CD) and uracil phosphoribosyltransferase (UPRT) enzymes. The opportunistic, intracellular pathoge...
Autores principales: | van der Geize, R., de Jong, W., Hessels, G. I., Grommen, A. W. F., Jacobs, A. A. C., Dijkhuizen, L. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2602762/ https://www.ncbi.nlm.nih.gov/pubmed/18984616 http://dx.doi.org/10.1093/nar/gkn811 |
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