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Identification and recovery of minor HIV-1 variants using the heteroduplex tracking assay and biotinylated probes
We describe a method to identify and recover minor human immunodeficiency virus type 1 (HIV-1) sequence variants from a complex population. The original heteroduplex tracking assay (HTA) was modified by incorporating a biotin tag into the probe to allow for direct sequence determination of the query...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2602764/ https://www.ncbi.nlm.nih.gov/pubmed/18948297 http://dx.doi.org/10.1093/nar/gkn713 |