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Identification and recovery of minor HIV-1 variants using the heteroduplex tracking assay and biotinylated probes

We describe a method to identify and recover minor human immunodeficiency virus type 1 (HIV-1) sequence variants from a complex population. The original heteroduplex tracking assay (HTA) was modified by incorporating a biotin tag into the probe to allow for direct sequence determination of the query...

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Detalles Bibliográficos
Autores principales: Schnell, Gretja, Ince, William L., Swanstrom, Ronald
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2602764/
https://www.ncbi.nlm.nih.gov/pubmed/18948297
http://dx.doi.org/10.1093/nar/gkn713