Quantification of siRNA using competitive qPCR

We have developed a PCR-based short interfering RNA (siRNA) quantification method based on competition between siRNA and a homologous DNA primer for annealing to template DNA, avoiding the requirement for prior conversion of RNA to cDNA. Primers and probe were designed to amplify regions of the huma...

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Detalles Bibliográficos
Autores principales: Liu, Wei-li, Stevenson, Mark, Seymour, Leonard W., Fisher, Kerry D.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615599/
https://www.ncbi.nlm.nih.gov/pubmed/19004870
http://dx.doi.org/10.1093/nar/gkn903

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615599/
https://www.ncbi.nlm.nih.gov/pubmed/19004870
http://dx.doi.org/10.1093/nar/gkn903

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