Cargando…

Quantification of siRNA using competitive qPCR

We have developed a PCR-based short interfering RNA (siRNA) quantification method based on competition between siRNA and a homologous DNA primer for annealing to template DNA, avoiding the requirement for prior conversion of RNA to cDNA. Primers and probe were designed to amplify regions of the huma...

Descripción completa

Detalles Bibliográficos
Autores principales:	Liu, Wei-li, Stevenson, Mark, Seymour, Leonard W., Fisher, Kerry D.
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2009
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615599/ https://www.ncbi.nlm.nih.gov/pubmed/19004870 http://dx.doi.org/10.1093/nar/gkn903

Ejemplares similares

Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification
por: Androvic, Peter, et al.
Publicado: (2017)

A bi-functional siRNA construct induces RNA interference and also primes PCR amplification for its own quantification
por: Jiang, Ming, et al.
Publicado: (2005)

Thermodynamic instability of siRNA duplex is a prerequisite for dependable prediction of siRNA activities
por: Ichihara, Masatoshi, et al.
Publicado: (2007)

OUHP: an optimized universal hairpin primer system for cost-effective and high-throughput RT-qPCR-based quantification of microRNA (miRNA) expression
por: He, Fang, et al.
Publicado: (2021)

Aptamer mediated siRNA delivery
por: Chu, Ted C., et al.
Publicado: (2006)

Surveillance of siRNA integrity by FRET imaging
por: Järve, Anne, et al.
Publicado: (2007)

High-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNA
por: Mestdagh, Pieter, et al.
Publicado: (2008)

Tetraalkylammonium derivatives as real-time PCR enhancers and stabilizers of the qPCR mixtures containing SYBR Green I
por: Shaik, Gouse M., et al.
Publicado: (2008)

Correction of RT–qPCR data for genomic DNA-derived signals with ValidPrime
por: Laurell, Henrik, et al.
Publicado: (2012)

ΩqPCR measures telomere length from single-cells in base pair units
por: Xiong, Fusheng, et al.
Publicado: (2021)

Analysis of siRNA specificity on targets with double-nucleotide mismatches
por: Dahlgren, Cecilia, et al.
Publicado: (2008)

An improved RT-qPCR method for direct quantification of enveloped RNA viruses
por: Gregorova, Pavlina, et al.
Publicado: (2022)

Impartial comparative analysis of measurement of leukocyte telomere length/DNA content by Southern blots and qPCR
por: Aviv, Abraham, et al.
Publicado: (2011)

Solid polymeric microparticles enhance the delivery of siRNA to macrophages in vivo
por: Lee, Sungmun, et al.
Publicado: (2009)

Quantification of microRNA editing using two-tailed RT-qPCR for improved biomarker discovery
por: Voss, Gjendine, et al.
Publicado: (2021)

RNA interference by mixtures of siRNAs prepared using custom oligonucleotide arrays
por: Oleinikov, Andrew V., et al.
Publicado: (2005)

siRNA target site secondary structure predictions using local stable substructures
por: Heale, Bret S. E., et al.
Publicado: (2005)

Selection of hyperfunctional siRNAs with improved potency and specificity
por: Wang, Xiaowei, et al.
Publicado: (2009)

RIsearch2: suffix array-based large-scale prediction of RNA–RNA interactions and siRNA off-targets
por: Alkan, Ferhat, et al.
Publicado: (2017)

Comparison of approaches for rational siRNA design leading to a new efficient and transparent method
por: Matveeva, Olga, et al.
Publicado: (2007)

Specific residues at every third position of siRNA shape its efficient RNAi activity
por: Katoh, Takayuki, et al.
Publicado: (2007)

Direct elicitation of template concentration from quantification cycle (C(q)) distributions in digital PCR
por: Mojtahedi, Mitra, et al.
Publicado: (2014)

Single-pot enzymatic synthesis of Dicer-substrate siRNAs
por: Guiley, Keelan Z., et al.
Publicado: (2012)

siSPOTR: a tool for designing highly specific and potent siRNAs for human and mouse
por: Boudreau, Ryan L., et al.
Publicado: (2013)

Carbodiimide-mediated cross-linking of RNA to nylon membranes improves the detection of siRNA, miRNA and piRNA by northern blot
por: Pall, Gurman Singh, et al.
Publicado: (2007)

Thorough validation of siRNA-induced cell death phenotypes defines new anti-apoptotic protein
por: Wu, Weilin, et al.
Publicado: (2006)

The importance of RT-qPCR primer design for the detection of siRNA-mediated mRNA silencing
por: Herbert, Mike, et al.
Publicado: (2011)

Detection of siRNA induced mRNA silencing by RT-qPCR: considerations for experimental design
por: Holmes, Katherine, et al.
Publicado: (2010)

Detection of siRNA administered to cells and animals by using a fluorescence intensity distribution analysis polarization system
por: Abe, Takashi, et al.
Publicado: (2009)

Cytoplasmic expression systems triggered by mRNA yield increased gene expression in post-mitotic neurons
por: Farrow, Paul J., et al.
Publicado: (2006)

Innovative microRNA quantification by qPCR
por: Le, Minh Ngoc, et al.
Publicado: (2023)

Multiplexing siRNAs to compress RNAi-based screen size in human cells
por: Martin, Scott E., et al.
Publicado: (2007)

siRNA nanoformulation against the Ret/PTC1 junction oncogene is efficient in an in vivo model of papillary thyroid carcinoma
por: de Martimprey, Henri, et al.
Publicado: (2008)

A novel and universal method for microRNA RT-qPCR data normalization
por: Mestdagh, Pieter, et al.
Publicado: (2009)

Simultaneous quantification of the most common and proteolytic Pseudomonas species in raw milk by multiplex qPCR
por: Maier, Christopher, et al.
Publicado: (2021)

A rapid and sensitive method to detect siRNA-mediated mRNA cleavage in vivo using 5′ RACE and a molecular beacon probe
por: Lasham, Annette, et al.
Publicado: (2010)

Detection of Cytoplasmic and Nuclear Circular RNA via RT-qPCR
por: Tan, Ke-En, et al.
Publicado: (2023)

Forward and robust selection of the most potent and noncellular toxic siRNAs from RNAi libraries
por: Li, Zhen, et al.
Publicado: (2009)

LORD-Q: a long-run real-time PCR-based DNA-damage quantification method for nuclear and mitochondrial genome analysis
por: Lehle, Simon, et al.
Publicado: (2014)

Roadblock-qPCR: a simple and inexpensive strategy for targeted measurements of mRNA stability
por: Watson, Maegan J., et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_3jd2t0bktf2o8iv25df5nh21uj