Forward and robust selection of the most potent and noncellular toxic siRNAs from RNAi libraries

Use of highly potent small interfering RNAs (siRNAs) can substantially reduce dose-dependent cytotoxic and off-target effects. We developed a genetic forward approach by fusing the cytosine deaminase gene with targets for the robust identification of highly potent siRNAs from RNA interference (RNAi)...

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Detalles Bibliográficos
Autores principales: Li, Zhen, Fortin, Yves, Shen, Shi-Hsiang
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615601/
https://www.ncbi.nlm.nih.gov/pubmed/19043072
http://dx.doi.org/10.1093/nar/gkn953

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615601/
https://www.ncbi.nlm.nih.gov/pubmed/19043072
http://dx.doi.org/10.1093/nar/gkn953

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