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A sequence-independent in vitro transposon-based strategy for efficient cloning of genomes of large DNA viruses as bacterial artificial chromosomes

Bacterial artificial chromosomes (BACs) derived from genomes of large DNA viruses are powerful tools for functional delineation of viral genes. Current methods for cloning the genomes of large DNA viruses as BACs require prior knowledge of the viral sequences or the cloning of viral DNA fragments, a...

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Detalles Bibliográficos
Autores principales: Zhou, Fuchun, Li, Qiuhua, Gao, Shou-Jiang
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615602/
https://www.ncbi.nlm.nih.gov/pubmed/18988631
http://dx.doi.org/10.1093/nar/gkn890