Improvement of bacterial transformation efficiency using plasmid artificial modification

We have developed a method to improve the transformation efficiency in genome-sequenced bacteria, using ‘Plasmid Artificial Modification’ (PAM), using the host's own restriction system. In this method, a shuttle vector was pre-methylated in Escherichia coli cells, which carry all the putative g...

Descripción completa

Detalles Bibliográficos
Autores principales: Yasui, Kazumasa, Kano, Yasunobu, Tanaka, Kaori, Watanabe, Kunitomo, Shimizu-Kadota, Mariko, Yoshikawa, Hirofumi, Suzuki, Tohru
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615632/
https://www.ncbi.nlm.nih.gov/pubmed/19004868
http://dx.doi.org/10.1093/nar/gkn884

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615632/
https://www.ncbi.nlm.nih.gov/pubmed/19004868
http://dx.doi.org/10.1093/nar/gkn884

Ejemplares similares