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Application of dissociation curve analysis to radiation hybrid panel marker scoring: generation of a map of river buffalo (B. bubalis) chromosome 20

BACKGROUND: Fluorescence of dyes bound to double-stranded PCR products has been utilized extensively in various real-time quantitative PCR applications, including post-amplification dissociation curve analysis, or differentiation of amplicon length or sequence composition. Despite the current era of...

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Detalles Bibliográficos
Autores principales: Kochan, Kelli J, Amaral, M Elisabete J, Agarwala, Richa, Schäffer, Alejandro A, Riggs, Penny K
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2621213/
https://www.ncbi.nlm.nih.gov/pubmed/19014630
http://dx.doi.org/10.1186/1471-2164-9-544