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A simplified, robust, and streamlined procedure for the production of C. elegans transgenes via recombineering
BACKGROUND: The nematode Caenorhabditis elegans has emerged as a powerful system to study biologic questions ranging from development to aging. The generation of transgenic animals is an important experimental tool and allows use of GFP fusion proteins to study the expression of genes of interest or...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2629773/ https://www.ncbi.nlm.nih.gov/pubmed/19116030 http://dx.doi.org/10.1186/1471-213X-8-119 |