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A simplified, robust, and streamlined procedure for the production of C. elegans transgenes via recombineering

BACKGROUND: The nematode Caenorhabditis elegans has emerged as a powerful system to study biologic questions ranging from development to aging. The generation of transgenic animals is an important experimental tool and allows use of GFP fusion proteins to study the expression of genes of interest or...

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Detalles Bibliográficos
Autores principales: Zhang, Yue, Nash, Lindsey, Fisher, Alfred L
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2629773/
https://www.ncbi.nlm.nih.gov/pubmed/19116030
http://dx.doi.org/10.1186/1471-213X-8-119