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A comprehensive collection of experimentally validated primers for Polymerase Chain Reaction quantitation of murine transcript abundance
BACKGROUND: Quantitative polymerase chain reaction (QPCR) is a widely applied analytical method for the accurate determination of transcript abundance. Primers for QPCR have been designed on a genomic scale but non-specific amplification of non-target genes has frequently been a problem. Although se...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2631021/ https://www.ncbi.nlm.nih.gov/pubmed/19108745 http://dx.doi.org/10.1186/1471-2164-9-633 |