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Transposition-Based Method for the Rapid Generation of Gene-Targeting Vectors to Produce Cre/Flp-Modifiable Conditional Knock-Out Mice

Conditional gene targeting strategies are progressively used to study gene function tissue-specifically and/or at a defined time period. Instrumental to all of these strategies is the generation of targeting vectors, and any methodology that would streamline the procedure would be highly beneficial....

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Detalles Bibliográficos
Autores principales: Turakainen, Hilkka, Saarimäki-Vire, Jonna, Sinjushina, Natalia, Partanen, Juha, Savilahti, Harri
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2632748/
https://www.ncbi.nlm.nih.gov/pubmed/19194496
http://dx.doi.org/10.1371/journal.pone.0004341