Novel mutations in Moloney Murine Leukemia Virus reverse transcriptase increase thermostability through tighter binding to template-primer

In an effort to increase the thermostability of Moloney Murine Leukemia Virus reverse transcriptase (MMLV RT), we screened random and site-saturation libraries for variants that show increased resistance to thermal inactivation. We discovered five mutations E69K, E302R, W313F, L435G and N454K that c...

Descripción completa

Detalles Bibliográficos
Autores principales: Arezi, Bahram, Hogrefe, Holly
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2632894/
https://www.ncbi.nlm.nih.gov/pubmed/19056821
http://dx.doi.org/10.1093/nar/gkn952

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2632894/
https://www.ncbi.nlm.nih.gov/pubmed/19056821
http://dx.doi.org/10.1093/nar/gkn952

Ejemplares similares