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Novel mutations in Moloney Murine Leukemia Virus reverse transcriptase increase thermostability through tighter binding to template-primer

In an effort to increase the thermostability of Moloney Murine Leukemia Virus reverse transcriptase (MMLV RT), we screened random and site-saturation libraries for variants that show increased resistance to thermal inactivation. We discovered five mutations E69K, E302R, W313F, L435G and N454K that c...

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Detalles Bibliográficos
Autores principales:	Arezi, Bahram, Hogrefe, Holly
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2009
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2632894/ https://www.ncbi.nlm.nih.gov/pubmed/19056821 http://dx.doi.org/10.1093/nar/gkn952

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