An unnatural base pair system for efficient PCR amplification and functionalization of DNA molecules

Toward the expansion of the genetic alphabet, we present an unnatural base pair system for efficient PCR amplification, enabling the site-specific incorporation of extra functional components into DNA. This system can be applied to conventional PCR protocols employing DNA templates containing unnatu...

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Detalles Bibliográficos
Autores principales: Kimoto, Michiko, Kawai, Rie, Mitsui, Tsuneo, Yokoyama, Shigeyuki, Hirao, Ichiro
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2632903/
https://www.ncbi.nlm.nih.gov/pubmed/19073696
http://dx.doi.org/10.1093/nar/gkn956

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2632903/
https://www.ncbi.nlm.nih.gov/pubmed/19073696
http://dx.doi.org/10.1093/nar/gkn956

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