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An unnatural base pair system for efficient PCR amplification and functionalization of DNA molecules
Toward the expansion of the genetic alphabet, we present an unnatural base pair system for efficient PCR amplification, enabling the site-specific incorporation of extra functional components into DNA. This system can be applied to conventional PCR protocols employing DNA templates containing unnatu...
Autores principales: | Kimoto, Michiko, Kawai, Rie, Mitsui, Tsuneo, Yokoyama, Shigeyuki, Hirao, Ichiro |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2632903/ https://www.ncbi.nlm.nih.gov/pubmed/19073696 http://dx.doi.org/10.1093/nar/gkn956 |
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