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pBT, a novel vector for tetracycline-regulated yeast three-hybrid assay
A novel yeast three-hybrid (Y3H) vector pBT was developed, which contains a tetracycline (Tet)-sensitive transactivator (tTA) expression unit and a Tet-responsive element (TRE)-driven 3rd protein expression unit within a single plasmid. To optimize tTA expression levels, several promoters for drivin...
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| Formato: | Texto |
| Lenguaje: | English |
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Oxford University Press
2009
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2632908/ https://www.ncbi.nlm.nih.gov/pubmed/19050013 http://dx.doi.org/10.1093/nar/gkn969 |