A single-step method for purification of active His-tagged ribosomes from a genetically engineered Escherichia coli

With the rapid development of the ribosome field in recent years a quick, simple and high-throughput method for purification of the bacterial ribosome is in demand. We have designed a new strain of Escherichia coli (JE28) by an in-frame fusion of a nucleotide sequence encoding a hexa-histidine affin...

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Detalles Bibliográficos
Autores principales: Ederth, Josefine, Mandava, Chandra Sekhar, Dasgupta, Santanu, Sanyal, Suparna
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2632923/
https://www.ncbi.nlm.nih.gov/pubmed/19074194
http://dx.doi.org/10.1093/nar/gkn992

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2632923/
https://www.ncbi.nlm.nih.gov/pubmed/19074194
http://dx.doi.org/10.1093/nar/gkn992

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