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A single-step method for purification of active His-tagged ribosomes from a genetically engineered Escherichia coli
With the rapid development of the ribosome field in recent years a quick, simple and high-throughput method for purification of the bacterial ribosome is in demand. We have designed a new strain of Escherichia coli (JE28) by an in-frame fusion of a nucleotide sequence encoding a hexa-histidine affin...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2632923/ https://www.ncbi.nlm.nih.gov/pubmed/19074194 http://dx.doi.org/10.1093/nar/gkn992 |