A pathway sensor for genome-wide screens of intracellular proteolytic cleavage

Protein cleavage is a central event in many regulated biological processes. We describe a system for detecting intracellular proteolysis based on non-conventional secretion of Gaussia luciferase (GLUC). GLUC exits the cell without benefit of a secretory leader peptide, but can be anchored in the cel...

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Detalles Bibliográficos
Autores principales: Ketteler, Robin, Sun, Zairen, Kovacs, Karl F, He, Wei-Wu, Seed, Brian
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2643935/
https://www.ncbi.nlm.nih.gov/pubmed/18387192
http://dx.doi.org/10.1186/gb-2008-9-4-r64
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author Ketteler, Robin
Sun, Zairen
Kovacs, Karl F
He, Wei-Wu
Seed, Brian
author_facet Ketteler, Robin
Sun, Zairen
Kovacs, Karl F
He, Wei-Wu
Seed, Brian
author_sort Ketteler, Robin
collection PubMed
description Protein cleavage is a central event in many regulated biological processes. We describe a system for detecting intracellular proteolysis based on non-conventional secretion of Gaussia luciferase (GLUC). GLUC exits the cell without benefit of a secretory leader peptide, but can be anchored in the cell by fusion to β-actin. By including protease cleavage sites between GLUC and β-actin, proteolytic cleavage can be detected. Using this assay, we have identified regulators of autophagy, apoptosis and β-actin cleavage.
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spelling pubmed-26439352009-02-17 A pathway sensor for genome-wide screens of intracellular proteolytic cleavage Ketteler, Robin Sun, Zairen Kovacs, Karl F He, Wei-Wu Seed, Brian Genome Biol Method Protein cleavage is a central event in many regulated biological processes. We describe a system for detecting intracellular proteolysis based on non-conventional secretion of Gaussia luciferase (GLUC). GLUC exits the cell without benefit of a secretory leader peptide, but can be anchored in the cell by fusion to β-actin. By including protease cleavage sites between GLUC and β-actin, proteolytic cleavage can be detected. Using this assay, we have identified regulators of autophagy, apoptosis and β-actin cleavage. BioMed Central 2008-04-03 /pmc/articles/PMC2643935/ /pubmed/18387192 http://dx.doi.org/10.1186/gb-2008-9-4-r64 Text en Copyright © 2008 Ketteler et al.; licensee BioMed Central Ltd. https://creativecommons.org/licenses/by/2.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0 (https://creativecommons.org/licenses/by/2.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Method
Ketteler, Robin
Sun, Zairen
Kovacs, Karl F
He, Wei-Wu
Seed, Brian
A pathway sensor for genome-wide screens of intracellular proteolytic cleavage
title A pathway sensor for genome-wide screens of intracellular proteolytic cleavage
title_full A pathway sensor for genome-wide screens of intracellular proteolytic cleavage
title_fullStr A pathway sensor for genome-wide screens of intracellular proteolytic cleavage
title_full_unstemmed A pathway sensor for genome-wide screens of intracellular proteolytic cleavage
title_short A pathway sensor for genome-wide screens of intracellular proteolytic cleavage
title_sort pathway sensor for genome-wide screens of intracellular proteolytic cleavage
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2643935/
https://www.ncbi.nlm.nih.gov/pubmed/18387192
http://dx.doi.org/10.1186/gb-2008-9-4-r64
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