Combining RNA Interference Mutants and Comparative Proteomics to Identify Protein Components and Dependences in a Eukaryotic Flagellum

Eukaryotic flagella from organisms such as Trypanosoma brucei can be isolated and their protein components identified by mass spectrometry. Here we used a comparative approach utilizing two-dimensional difference gel electrophoresis and isobaric tags for relative and absolute quantitation to reveal...

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Detalles Bibliográficos
Autores principales: Portman, Neil, Lacomble, Sylvain, Thomas, Benjamin, McKean, Paul G., Gull, Keith
Formato: Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2009
Materias:
Genomics, Proteomics, and Bioinformatics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2645819/
https://www.ncbi.nlm.nih.gov/pubmed/19074134
http://dx.doi.org/10.1074/jbc.M808859200

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2645819/
https://www.ncbi.nlm.nih.gov/pubmed/19074134
http://dx.doi.org/10.1074/jbc.M808859200

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