Cargando…

Combining RNA Interference Mutants and Comparative Proteomics to Identify Protein Components and Dependences in a Eukaryotic Flagellum

Eukaryotic flagella from organisms such as Trypanosoma brucei can be isolated and their protein components identified by mass spectrometry. Here we used a comparative approach utilizing two-dimensional difference gel electrophoresis and isobaric tags for relative and absolute quantitation to reveal...

Descripción completa

Detalles Bibliográficos
Autores principales:	Portman, Neil, Lacomble, Sylvain, Thomas, Benjamin, McKean, Paul G., Gull, Keith
Formato:	Texto
Lenguaje:	English
Publicado:	American Society for Biochemistry and Molecular Biology 2009
Materias:	Genomics, Proteomics, and Bioinformatics
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2645819/ https://www.ncbi.nlm.nih.gov/pubmed/19074134 http://dx.doi.org/10.1074/jbc.M808859200

Ejemplares similares

A Protein-Protein Interaction Map of the Trypanosoma brucei Paraflagellar Rod
por: Lacomble, Sylvain, et al.
Publicado: (2009)

A Trypanosoma brucei Protein Required for Maintenance of the Flagellum Attachment Zone and Flagellar Pocket ER Domains
por: Lacomble, Sylvain, et al.
Publicado: (2012)

Discovery Proteomics Identifies a Molecular Link between the Coatomer Protein Complex I and Androgen Receptor-dependent Transcription
por: Hsiao, Jordy J., et al.
Publicado: (2016)

Disulfide Bond Formation and Cysteine Exclusion in Gram-positive Bacteria
por: Daniels, Robert, et al.
Publicado: (2010)

Angiotensin-converting Enzyme Is a Modifier of Hypertensive End Organ Damage
por: Liu, Xiaojun, et al.
Publicado: (2009)

The Butyrylcholinesterase K Variant Confers Structurally Derived Risks for Alzheimer Pathology(♦)
por: Podoly, Erez, et al.
Publicado: (2009)

mRNA Display Design of Fibronectin-based Intrabodies That Detect and Inhibit Severe Acute Respiratory Syndrome Coronavirus Nucleocapsid Protein
por: Liao, Hsiang-I., et al.
Publicado: (2009)

Two C-type Lectins Cooperate to Defend Anopheles gambiae against Gram-negative Bacteria
por: Schnitger, Anna K. D., et al.
Publicado: (2009)

A Common Mutation in Paraoxonase-2 Results in Impaired Lactonase Activity
por: Stoltz, David A., et al.
Publicado: (2009)

Response of Gastric Epithelial Progenitors to Helicobacter pylori Isolates Obtained from Swedish Patients with Chronic Atrophic Gastritis
por: Giannakis, Marios, et al.
Publicado: (2009)

The Autocatalytic Release of a Putative RNA Virus Transcription Factor from Its Polyprotein Precursor Involves Two Paralogous Papain-like Proteases That Cleave the Same Peptide Bond
por: Ziebuhr, John, et al.
Publicado: (2001)

Functional Gene Expression Profiling in Yeast Implicates Translational Dysfunction in Mutant Huntingtin Toxicity
por: Tauber, Eran, et al.
Publicado: (2011)

Proteomic Characterization of Plasma Membrane-proximal T Cell Activation Responses
por: de Wet, Ben, et al.
Publicado: (2011)

Identification of New Substrates of the Protein-tyrosine Phosphatase PTP1B by Bayesian Integration of Proteome Evidence
por: Ferrari, Emanuela, et al.
Publicado: (2011)

Proteomic Analysis Reveals Age-related Changes in Tendon Matrix Composition, with Age- and Injury-specific Matrix Fragmentation
por: Peffers, Mandy J., et al.
Publicado: (2014)

Proteomic Analysis of Native Hepatocyte Nuclear Factor-4α (HNF4α) Isoforms, Phosphorylation Status, and Interactive Cofactors
por: Daigo, Kenji, et al.
Publicado: (2011)

Microarray and Proteomic Analysis of Breast Cancer Cell and Osteoblast Co-cultures: ROLE OF OSTEOBLAST MATRIX METALLOPROTEINASE (MMP)-13 IN BONE METASTASIS
por: Morrison, Charlotte, et al.
Publicado: (2011)

Genome-wide Analysis of Transcription Factor E2F1 Mutant Proteins Reveals That N- and C-terminal Protein Interaction Domains Do Not Participate in Targeting E2F1 to the Human Genome
por: Cao, Alina R., et al.
Publicado: (2011)

Data-independent proteome analysis of ARPE-19 cells
por: Koirala, Diwa, et al.
Publicado: (2018)

Dataset on fat body proteome of Anopheles stephensi Liston
por: Kumar, Manish, et al.
Publicado: (2019)

Proteomic dataset of wolframin-deficient mouse heart and skeletal muscles
por: Eimre, Margus, et al.
Publicado: (2018)

Data set of the proteome of fresh and frozen thawed stallion spermatozoa
por: Martín-Cano, Francisco E., et al.
Publicado: (2020)

Proteomic datasets of uninfected and Staphylococcus aureus-infected goat milk
por: Pisanu, Salvatore, et al.
Publicado: (2020)

Mass Spectrometry Imaging and Identification of Peptides Associated with Cephalic Ganglia Regeneration in Schmidtea mediterranea
por: Ong, Ta-Hsuan, et al.
Publicado: (2016)

Interactome Analyses of Mature γ-Secretase Complexes Reveal Distinct Molecular Environments of Presenilin (PS) Paralogs and Preferential Binding of Signal Peptide Peptidase to PS2
por: Jeon, Amy Hye Won, et al.
Publicado: (2013)

A proteomic dataset of secreted proteins by three Staphylococcus saprophyticus strains
por: Oliveira, Andrea Santana de, et al.
Publicado: (2018)

High-throughput mass spectrometry and bioinformatics analysis of breast cancer proteomic data
por: Gomig, Talita Helen Bombardelli, et al.
Publicado: (2019)

Proteome data to explore the axolotl limb regeneration capacity at neotenic and metamorphic stages
por: Demircan, Turan, et al.
Publicado: (2020)

Shotgun proteomics datasets acquired on Gammarus pulex animals sampled from the wild
por: Gouveia, Duarte, et al.
Publicado: (2019)

SARS-CoV-2 (COVID-19) structural and evolutionary dynamicome: Insights into functional evolution and human genomics
por: Gupta, Ruchir, et al.
Publicado: (2020)

The Plasma Proteome Is Associated with Anthropometric Status of Undernourished Nepalese School-Aged Children(1)(2)(3)
por: Lee, Sun Eun, et al.
Publicado: (2017)

Data on proteomic profiling of cells and extracellular vesicles of the melittin-resistant Acholeplasma laidlawii strain
por: Medvedeva, E.S., et al.
Publicado: (2019)

Human tear fluid proteome dataset for usage as a spectral library and for protein modeling
por: Guntermann, Annika, et al.
Publicado: (2019)

Data from differentially expressed proteins in platelet components associated with adverse transfusion reactions
por: Aloui, Chaker, et al.
Publicado: (2019)

Hyperosmolality in CHO cell culture: effects on the proteome
por: Romanova, Nadiya, et al.
Publicado: (2022)

Modulation of flagellum attachment zone protein FLAM3 and regulation of the cell shape in Trypanosoma brucei life cycle transitions
por: Sunter, Jack D., et al.
Publicado: (2015)

Spiked human substantia nigra proteome data set for use as a spectral library for protein modelling and protein mapping
por: Steinbach, Simone, et al.
Publicado: (2019)

Data on proteomic profiling of extracellular vesicles of Acholeplasma laidlawii strains with increased resistance to antibiotics of different classes - ciprofloxacin and tetracycline
por: Mouzykantov, Alexey, et al.
Publicado: (2020)

Exploration of human cerebrospinal fluid: A large proteome dataset revealed by trapped ion mobility time-of-flight mass spectrometry
por: Macron, Charlotte, et al.
Publicado: (2020)

A proteomic profiling dataset of recombinant Chinese hamster ovary cells showing enhanced cellular growth following miR-378 depletion
por: Coleman, Orla, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_lpdmhv0emv952nntfrj5q2h9db