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Combining RNA Interference Mutants and Comparative Proteomics to Identify Protein Components and Dependences in a Eukaryotic Flagellum

Eukaryotic flagella from organisms such as Trypanosoma brucei can be isolated and their protein components identified by mass spectrometry. Here we used a comparative approach utilizing two-dimensional difference gel electrophoresis and isobaric tags for relative and absolute quantitation to reveal...

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Detalles Bibliográficos
Autores principales:	Portman, Neil, Lacomble, Sylvain, Thomas, Benjamin, McKean, Paul G., Gull, Keith
Formato:	Texto
Lenguaje:	English
Publicado:	American Society for Biochemistry and Molecular Biology 2009
Materias:	Genomics, Proteomics, and Bioinformatics
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2645819/ https://www.ncbi.nlm.nih.gov/pubmed/19074134 http://dx.doi.org/10.1074/jbc.M808859200

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