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Intrakinetochore stretch is associated with changes in kinetochore phosphorylation and spindle assembly checkpoint activity
Cells have evolved a signaling pathway called the spindle assembly checkpoint (SAC) to increase the fidelity of chromosome segregation by generating a “wait anaphase” signal until all chromosomes are properly aligned within the mitotic spindle. It has been proposed that tension generated by the stre...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2646557/ https://www.ncbi.nlm.nih.gov/pubmed/19193623 http://dx.doi.org/10.1083/jcb.200808130 |
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author | Maresca, Thomas J. Salmon, Edward D. |
author_facet | Maresca, Thomas J. Salmon, Edward D. |
author_sort | Maresca, Thomas J. |
collection | PubMed |
description | Cells have evolved a signaling pathway called the spindle assembly checkpoint (SAC) to increase the fidelity of chromosome segregation by generating a “wait anaphase” signal until all chromosomes are properly aligned within the mitotic spindle. It has been proposed that tension generated by the stretch of the centromeric chromatin of bioriented chromosomes stabilizes kinetochore microtubule attachments and turns off SAC activity. Although biorientation clearly causes stretching of the centromeric chromatin, it is unclear whether the kinetochore is also stretched. To test whether intrakinetochore stretch occurs and is involved in SAC regulation, we developed a Drosophila melanogaster S2 cell line expressing centromere identifier–mCherry and Ndc80–green fluorescent protein to mark the inner and outer kinetochore domains, respectively. We observed stretching within kinetochores of bioriented chromosomes by monitoring both inter- and intrakinetochore distances in live cell assays. This intrakinetochore stretch is largely independent of a 30-fold variation in centromere stretch. Furthermore, loss of intrakinetochore stretch is associated with enhancement of 3F3/2 phosphorylation and SAC activation. |
format | Text |
id | pubmed-2646557 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-26465572009-08-09 Intrakinetochore stretch is associated with changes in kinetochore phosphorylation and spindle assembly checkpoint activity Maresca, Thomas J. Salmon, Edward D. J Cell Biol Research Articles Cells have evolved a signaling pathway called the spindle assembly checkpoint (SAC) to increase the fidelity of chromosome segregation by generating a “wait anaphase” signal until all chromosomes are properly aligned within the mitotic spindle. It has been proposed that tension generated by the stretch of the centromeric chromatin of bioriented chromosomes stabilizes kinetochore microtubule attachments and turns off SAC activity. Although biorientation clearly causes stretching of the centromeric chromatin, it is unclear whether the kinetochore is also stretched. To test whether intrakinetochore stretch occurs and is involved in SAC regulation, we developed a Drosophila melanogaster S2 cell line expressing centromere identifier–mCherry and Ndc80–green fluorescent protein to mark the inner and outer kinetochore domains, respectively. We observed stretching within kinetochores of bioriented chromosomes by monitoring both inter- and intrakinetochore distances in live cell assays. This intrakinetochore stretch is largely independent of a 30-fold variation in centromere stretch. Furthermore, loss of intrakinetochore stretch is associated with enhancement of 3F3/2 phosphorylation and SAC activation. The Rockefeller University Press 2009-02-09 /pmc/articles/PMC2646557/ /pubmed/19193623 http://dx.doi.org/10.1083/jcb.200808130 Text en © 2009 Maresca and Salmon This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Maresca, Thomas J. Salmon, Edward D. Intrakinetochore stretch is associated with changes in kinetochore phosphorylation and spindle assembly checkpoint activity |
title | Intrakinetochore stretch is associated with changes in kinetochore phosphorylation and spindle assembly checkpoint activity |
title_full | Intrakinetochore stretch is associated with changes in kinetochore phosphorylation and spindle assembly checkpoint activity |
title_fullStr | Intrakinetochore stretch is associated with changes in kinetochore phosphorylation and spindle assembly checkpoint activity |
title_full_unstemmed | Intrakinetochore stretch is associated with changes in kinetochore phosphorylation and spindle assembly checkpoint activity |
title_short | Intrakinetochore stretch is associated with changes in kinetochore phosphorylation and spindle assembly checkpoint activity |
title_sort | intrakinetochore stretch is associated with changes in kinetochore phosphorylation and spindle assembly checkpoint activity |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2646557/ https://www.ncbi.nlm.nih.gov/pubmed/19193623 http://dx.doi.org/10.1083/jcb.200808130 |
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