A versatile non-radioactive assay for DNA methyltransferase activity and DNA binding

We present a simple, non-radioactive assay for DNA methyltransferase activity and DNA binding. As most proteins are studied as GFP fusions in living cells, we used a GFP binding nanobody coupled to agarose beads (GFP nanotrap) for rapid one-step purification. Immobilized GFP fusion proteins were sub...

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Detalles Bibliográficos
Autores principales: Frauer, Carina, Leonhardt, Heinrich
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2647308/
https://www.ncbi.nlm.nih.gov/pubmed/19129216
http://dx.doi.org/10.1093/nar/gkn1029

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2647308/
https://www.ncbi.nlm.nih.gov/pubmed/19129216
http://dx.doi.org/10.1093/nar/gkn1029

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