Sensing peptide–oligonucleotide interactions by a two-color fluorescence label: application to the HIV-1 nucleocapsid protein

We present a new methodology for site-specific sensing of peptide–oligonucleotide (ODN) interactions using a solvatochromic fluorescent label based on 3-hydroxychromone (3HC). This label was covalently attached to the N-terminus of a peptide corresponding to the zinc finger domain of the HIV-1 nucle...

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Detalles Bibliográficos
Autores principales: Shvadchak, Volodymyr V., Klymchenko, Andrey S., de Rocquigny, Hugues, Mély, Yves
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2647317/
https://www.ncbi.nlm.nih.gov/pubmed/19151084
http://dx.doi.org/10.1093/nar/gkn1083

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2647317/
https://www.ncbi.nlm.nih.gov/pubmed/19151084
http://dx.doi.org/10.1093/nar/gkn1083

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