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Sensitive isothermal detection of nucleic-acid sequence by primer generation–rolling circle amplification
A simple isothermal nucleic-acid amplification reaction, primer generation–rolling circle amplification (PG–RCA), was developed to detect specific nucleic-acid sequences of sample DNA. This amplification method is achievable at a constant temperature (e.g. 60°C) simply by mixing circular single-stra...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2647323/ https://www.ncbi.nlm.nih.gov/pubmed/19106144 http://dx.doi.org/10.1093/nar/gkn1014 |