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Sensitive isothermal detection of nucleic-acid sequence by primer generation–rolling circle amplification

A simple isothermal nucleic-acid amplification reaction, primer generation–rolling circle amplification (PG–RCA), was developed to detect specific nucleic-acid sequences of sample DNA. This amplification method is achievable at a constant temperature (e.g. 60°C) simply by mixing circular single-stra...

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Detalles Bibliográficos
Autores principales:	Murakami, Taku, Sumaoka, Jun, Komiyama, Makoto
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2009
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2647323/ https://www.ncbi.nlm.nih.gov/pubmed/19106144 http://dx.doi.org/10.1093/nar/gkn1014

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