A simplified in vitro ligation approach to clone an E1B55k-deleted double-targeted conditionally-replicative adenovirus

BACKGROUND: Construction of conditionally-replicative Adenovirus (CRAd) is complex and time-consuming. While homologous recombination (HR) using a two-plasmid system in bacteria is commonly used to generate CRAds, alternative methods may be required when HR fails. Previously, in vitro ligation has b...

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Detalles Bibliográficos
Autor principal: Haviv, Yosef S
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2647529/
https://www.ncbi.nlm.nih.gov/pubmed/19200390
http://dx.doi.org/10.1186/1743-422X-6-18

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2647529/
https://www.ncbi.nlm.nih.gov/pubmed/19200390
http://dx.doi.org/10.1186/1743-422X-6-18

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