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A simplified in vitro ligation approach to clone an E1B55k-deleted double-targeted conditionally-replicative adenovirus
BACKGROUND: Construction of conditionally-replicative Adenovirus (CRAd) is complex and time-consuming. While homologous recombination (HR) using a two-plasmid system in bacteria is commonly used to generate CRAds, alternative methods may be required when HR fails. Previously, in vitro ligation has b...
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| Formato: | Texto |
| Lenguaje: | English |
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BioMed Central
2009
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2647529/ https://www.ncbi.nlm.nih.gov/pubmed/19200390 http://dx.doi.org/10.1186/1743-422X-6-18 |