Cargando…

A Toolkit and Robust Pipeline for the Generation of Fosmid-Based Reporter Genes in C. elegans

Engineering fluorescent proteins into large genomic clones, contained within BACs or fosmid vectors, is a tool to visualize and study spatiotemporal gene expression patterns in transgenic animals. Because these reporters cover large genomic regions, they most likely capture all cis-regulatory inform...

Descripción completa

Detalles Bibliográficos
Autores principales: Tursun, Baris, Cochella, Luisa, Carrera, Inés, Hobert, Oliver
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2649505/
https://www.ncbi.nlm.nih.gov/pubmed/19259264
http://dx.doi.org/10.1371/journal.pone.0004625