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A Toolkit and Robust Pipeline for the Generation of Fosmid-Based Reporter Genes in C. elegans
Engineering fluorescent proteins into large genomic clones, contained within BACs or fosmid vectors, is a tool to visualize and study spatiotemporal gene expression patterns in transgenic animals. Because these reporters cover large genomic regions, they most likely capture all cis-regulatory inform...
Autores principales: | Tursun, Baris, Cochella, Luisa, Carrera, Inés, Hobert, Oliver |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2649505/ https://www.ncbi.nlm.nih.gov/pubmed/19259264 http://dx.doi.org/10.1371/journal.pone.0004625 |
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