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Simplified production and concentration of HIV-1-based lentiviral vectors using HYPERFlask vessels and anion exchange membrane chromatography

BACKGROUND: During the past twelve years, lentiviral (LV) vectors have emerged as valuable tools for transgene delivery because of their ability to transduce nondividing cells and their capacity to sustain long-term transgene expression in target cells in vitro and in vivo. However, despite signific...

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Detalles Bibliográficos
Autores principales: Kutner, Robert H, Puthli, Sharon, Marino, Michael P, Reiser, Jakob
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2649911/
https://www.ncbi.nlm.nih.gov/pubmed/19220915
http://dx.doi.org/10.1186/1472-6750-9-10