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Simplified production and concentration of HIV-1-based lentiviral vectors using HYPERFlask vessels and anion exchange membrane chromatography
BACKGROUND: During the past twelve years, lentiviral (LV) vectors have emerged as valuable tools for transgene delivery because of their ability to transduce nondividing cells and their capacity to sustain long-term transgene expression in target cells in vitro and in vivo. However, despite signific...
Autores principales: | Kutner, Robert H, Puthli, Sharon, Marino, Michael P, Reiser, Jakob |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2649911/ https://www.ncbi.nlm.nih.gov/pubmed/19220915 http://dx.doi.org/10.1186/1472-6750-9-10 |
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