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Exploration of Human ORFeome: High-Throughput Preparation of ORF Clones and Efficient Characterization of Their Protein Products

In this study, we established new systematic protocols for the preparation of cDNA clones, conventionally termed open reading frame (ORF) clones, suitable for characterization of their gene products by adopting a restriction-enzyme-assisted cloning method using the Flexi(®) cloning system. The syste...

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Detalles Bibliográficos
Autores principales: Nagase, Takahiro, Yamakawa, Hisashi, Tadokoro, Shinichi, Nakajima, Daisuke, Inoue, Shinichi, Yamaguchi, Kei, Itokawa, Yasuhide, Kikuno, Reiko F., Koga, Hisashi, Ohara, Osamu
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2650635/
https://www.ncbi.nlm.nih.gov/pubmed/18316326
http://dx.doi.org/10.1093/dnares/dsn004