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Exploration of Human ORFeome: High-Throughput Preparation of ORF Clones and Efficient Characterization of Their Protein Products
In this study, we established new systematic protocols for the preparation of cDNA clones, conventionally termed open reading frame (ORF) clones, suitable for characterization of their gene products by adopting a restriction-enzyme-assisted cloning method using the Flexi(®) cloning system. The syste...
Autores principales: | Nagase, Takahiro, Yamakawa, Hisashi, Tadokoro, Shinichi, Nakajima, Daisuke, Inoue, Shinichi, Yamaguchi, Kei, Itokawa, Yasuhide, Kikuno, Reiko F., Koga, Hisashi, Ohara, Osamu |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2650635/ https://www.ncbi.nlm.nih.gov/pubmed/18316326 http://dx.doi.org/10.1093/dnares/dsn004 |
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